Phytochemical screening and in vivo test of dewandaru (Eugenia uniflora L) fruit extract on mice exposed to cigarette smoke
Keywords:
cigarette smoke, Eugenia uniflora, fruit extract, MDAAbstract
Cigarette smoke contains various toxic chemical compounds which can trigger oxidative stress. Biomarkers to show the degree of oxidative stress that occurs are levels of malondialdehyde (MDA) The n-butanol extract of Eugenia uniflora fruit contains compounds that have antioxidant activity of phenolic and non-phenolic compounds. The purpose of this study was phytochemical screening and the effect of giving n-butanol extract of fruit Eugenia uniflora L on oxidative stress mice exposed to cigarette smoke. The research design was an experimental randomized post-test control group design, The control group of mice was only given exposure to cigarette smoke 1 stick per day (K), the second group was given exposure to cigarette smoke 1 stick per day P1 dose of 100 mg /KgBB, the third group was given exposure to cigarette smoke 1 stick per day P2 dose 200 mg /KgBB, n-butanol Dewandaru fruit extract. The treatment of experimental animals was given for 30 days, malondialdehyde was analyzed using the Elisa method.
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Abdul-Rasheed, O. F., & Al-Rubayee, W. T. (2013). Effects of cigarette smoking on lipid peroxidation and antioxidant status in Iraqi men at Baghdad city. International Journal of Basic and Applied Sciences, 2(1), 47.
Agati, G., Azzarello, E., Pollastri, S., & Tattini, M. (2012). Flavonoids as antioxidants in plants: location and functional significance. Plant science, 196, 67-76.
Bagetti, M., Facco, E. M. P., Piccolo, J., Hirsch, G. E., Rodriguez-Amaya, D., Kobori, C. N., ... & Emanuelli, T. (2011). Physicochemical characterization and antioxidant capacity of pitanga fruits (Eugenia uniflora L.). Food Science and Technology, 31, 147-154.
Band, P. R., Le, N. D., Fang, R., & Deschamps, M. (2002). Carcinogenic and endocrine disrupting effects of cigarette smoke and risk of breast cancer. The Lancet, 360(9339), 1044-1049. https://doi.org/10.1016/S0140-6736(02)11140-8
Birben, E., Sahiner, U. M., Sackesen, C., Erzurum, S., & Kalayci, O. (2012). Oxidative stress and antioxidant defense. World Allergy Organization Journal, 5(1), 9-19.
Celli, G. B., Pereira-Netto, A. B., & Beta, T. (2011). Comparative analysis of total phenolic content, antioxidant activity, and flavonoids profile of fruits from two varieties of Brazilian cherry (Eugenia uniflora L.) throughout the fruit developmental stages. Food research international, 44(8), 2442-2451.
Ergina, E., Nuryanti, S., & Pursitasari, I. D. (2014). Uji kualitatif senyawa metabolit sekunder pada daun palado (Agave angustifolia) yang diekstraksi dengan pelarut air dan etanol. Jurnal Akademika Kimia, 3(3), 165-172.
Hasday, J. D., Bascom, R., Costa, J. J., Fitzgerald, T., & Dubin, W. (1999). Bacterial endotoxin is an active component of cigarette smoke. Chest, 115(3), 829-835. https://doi.org/10.1378/chest.115.3.829
Janicka, M., Kot-Wasik, A., Kot, J., & Namie?nik, J. (2010). Isoprostanes-biomarkers of lipid peroxidation: their utility in evaluating oxidative stress and analysis. International Journal of Molecular Sciences, 11(11), 4631-4659.
Jeusette, I., Salas, A., Iraculis, N., Compagnucci, M., Torre, C., Romano, V., & Kirschvink, N. (2009). Increased urinary F2-isoprostane concentration as an indicator of oxidative stress in overweight cats. Blood, 92, 15.
Kiswandono, A. A. (2017). Skrining senyawa kimia dan pengaruh metode maserasi dan refluks pada biji kelor (moringa oleifera, lamk) terhadap rendemen ekstrak yang dihasilkan. Jurnal Sains Natural, 1(2), 126-134.
Kwak, H. G., & Lim, H. B. (2014). Inhibitory effects of Cnidium monnieri fruit extract on pulmonary inflammation in mice induced by cigarette smoke condensate and lipopolysaccharide. Chinese journal of natural medicines, 12(9), 641-647. https://doi.org/10.1016/S1875-5364(14)60098-4
Lykkesfeldt, J. (2007). Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clinica chimica acta, 380(1-2), 50-58. https://doi.org/10.1016/j.cca.2007.01.028
Moldoveanu, S. C., & Kiser, M. (2007). Gas chromatography/mass spectrometry versus liquid chromatography/fluorescence detection in the analysis of phenols in mainstream cigarette smoke. Journal of Chromatography A, 1141(1), 90-97. https://doi.org/10.1016/j.chroma.2006.11.100
Murray, A. B., & Morrison, B. J. (1993). The decrease in severity of asthma in children of parents who smoke since the parents have been exposing them to less cigarette smoke. Journal of allergy and clinical immunology, 91(1), 102-110. https://doi.org/10.1016/0091-6749(93)90302-V
Mustafa, R. A., Hamid, A. A., Mohamed, S., & Bakar, F. A. (2010). Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. Journal of food science, 75(1), C28-C35.
Oliveira, A. L., Lopes, R. B., Cabral, F. A., & Eberlin, M. N. (2006). Volatile compounds from pitanga fruit (Eugenia uniflora L.). Food Chemistry, 99(1), 1-5. https://doi.org/10.1016/j.foodchem.2005.07.012
Oliveira, P. S., Chaves, V. C., Bona, N. P., Soares, M. S. P., de Souza Cardoso, J., Vasconcellos, F. A., ... & Stefanello, F. M. (2017). Eugenia uniflora fruit (red type) standardized extract: a potential pharmacological tool to diet-induced metabolic syndrome damage management. Biomedicine & pharmacotherapy, 92, 935-941.
Onwudiwe, N. N., Njoku, O. U., & Joshua, P. E. (2010). Phytochemical Analysis and Acute Toxicity/Lethality Study of Ethanol Extract of Eugenia uniflora Pulp. Research Journal of Pharmacognosy and Phytochemistry, 2(4), 336-339.
Ozougwu, J. C. (2016). The role of reactive oxygen species and antioxidants in oxidative stress. International Journal of Research, 1.
Santoso, P., Sari, N. W. B., Yuda, P. E. S. K., & Wardani, I. G. A. A. K. (2018). Skrining Fitokimia dan Uji Aktivitas Antiinflamasi Ekstrak N-Butanol Buah Dewandaru (Eugenia Uniflora L.) pada Tikus Putih Jantan Galur Wistar (Rattus Norvegicus) dengan Metode Paw Edema yang Diinduksi Karagenan. Jurnal Ilmiah Medicamento, 4(2), 100-106.
Schapoval, E. E. S., Silveira, S. M., Miranda, M. L., Alice, C. B., & Henriques, A. T. (1994). Evaluation of some pharmacological activities of Eugenia uniflora L. Journal of ethnopharmacology, 44(3), 137-142. https://doi.org/10.1016/0378-8741(94)01178-8
Simaremare, E. S. (2014). Skrining fitokimia ekstrak etanol daun gatal (Laportea decumana (Roxb.) Wedd). PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 11(1).
Sopori, M. L., & Kozak, W. (1998). Immunomodulatory effects of cigarette smoke. Journal of neuroimmunology, 83(1-2), 148-156. https://doi.org/10.1016/S0165-5728(97)00231-2
Susanty, S., & Bachmid, F. (2016). Perbandingan metode ekstraksi maserasi dan refluks terhadap kadar fenolik dari ekstrak tongkol jagung (Zea mays L.). Jurnal Konversi, 5(2), 87-92.
Swan, G. E., & Lessov-Schlaggar, C. N. (2007). The effects of tobacco smoke and nicotine on cognition and the brain. Neuropsychology review, 17(3), 259-273.
Syakur, A. A., Rusdiawan, R., & Sukri, M. (2018). Text of cigarette advertisement: A semiology study of Roland Barthes. International journal of linguistics, literature and culture, 4(3), 72-79.
Tohomi, K. L. (2014). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Buasbuas (Premna Cordifolia Linn.) Terhadap Gambaran Histopatologi Paru Tikus (Rattus Norvegicus) Wistar Jantan Pasca Paparan Asap Rokok. Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 1(1).
Wibowo, D. V., Pangemanan, D. H., & Polii, H. (2017). Hubungan Merokok dengan Kadar Hemoglobin dan Trombosit pada Perokok Dewasa. eBiomedik, 5(2).
Wijaya, D. P., Paendong, J. E., & Abidjulu, J. (2014). Skrining fitokimia dan uji aktivitas antioksidan dari daun nasi (Phrynium capitatum) dengan metode DPPH (1, 1-difenil-2-pikrilhidrazil). Jurnal MIPA, 3(1), 11-15.
Winarsi, H. (2007). Natural Antioxidants and Free Radicals: Potential and Its Application in Health. Kanisius. Yogyakarta.
Wyatt, T. A., Kharbanda, K. K., McCaskill, M. L., Tuma, D. J., Yanov, D., DeVasure, J., & Sisson, J. H. (2012). Malondialdehyde–acetaldehyde-adducted protein inhalation causes lung injury. Alcohol, 46(1), 51-59. https://doi.org/10.1016/j.alcohol.2011.09.001
Yanbaeva, D. G., Dentener, M. A., Creutzberg, E. C., Wesseling, G., & Wouters, E. F. (2007). Systemic effects of smoking. Chest, 131(5), 1557-1566.
Yao, H., Sundar, I. K., Ahmad, T., Lerner, C., Gerloff, J., Friedman, A. E., ... & Rahman, I. (2014). SIRT1 protects against cigarette smoke-induced lung oxidative stress via a FOXO3-dependent mechanism. American Journal of Physiology-Lung Cellular and Molecular Physiology, 306(9), L816-L828.
Yoshida, Y., Umeno, A., & Shichiri, M. (2013). Lipid peroxidation biomarkers for evaluating oxidative stress and assessing antioxidant capacity in vivo. Journal of clinical biochemistry and nutrition, 52(1), 9-16.
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