Development of Collagen Peptide Raw Materials from Milkfish Scale Waste (Chanos Chanos) as a Bioactive Emulsifier
Keywords:
milkfish scales, collagen, emulsifying agentAbstract
Collagen peptides produced by milkfish (Chanos chanos) scales can be an alternative to replace collagen from mammals. Besides the high price of mammalian collagen, it also risks spreading infectious diseases like Bovine Spongiform Encephalopathy (BSE) and Transmissible spongiform encephalopathies (TSEs). This study aims to identify the effect of acetic acid and pepsin concentrations in optimizing the extraction of collagen peptides from milkfish scales due to waste and its application as a bioactive emulsifier. This study used an experimental design using a completely randomized design ( 3 x 2 factorial pattern. The results of this study on the proximate analysis of collagen peptides showed that the results of the moisture content test, ash content test, protein content test, fat content test, and pH test met the requirements for collagen quality. In testing the quality of the emulsifier, all groups met the criteria for homogeneity, viscosity, and percent transmittance where a concentration of 0.5 M with a variation of pepsin 0.10%, a concentration of 0.75 M with a variation of 0.05% pepsin, and a concentration of 0.75 M with a variation of pepsin 0.10% produces the most stable collagen in terms of Creaming Index and Measurement of visible layer boundaries during storage.
Downloads
References
Anonim. Bandeng presto karawang siap bersaing Diambil dari: https://megapolitan.antaranews.com/berita/38909/bandeng-presto-karawang-siap-bersaing, Diakses pada tanggal 17 Desember 2019.
Anwar HS, Antasari M, Hasni D, Sapriani N, Rohaya S, Winarti C. 2017. Kombinasi Pati Sukun Termodifikasi Osa (Octenyl Succinic Anhydride) Dan Lesitin Sebagai Penstabil Emulsi Minyak Dalam Air. Jurnal Penelitian Pascapanen Pertanian. 14(3) : 124 - 133.
AOAC. 2005. Official Methods of Analysis. Washington: Association of Official Analytical Chemists.
Arvanitoyannis IS, Kassaveti A. 2009. Starch-Cellulose Blends. DIdalam Yu L, editor. Biodegradable Polymer Blends and Composites from RenewablenResources. New Jersey : John Wiley. Hlm 19-54.
Aulton ME, Kevin MG, Taylor. 2001. Pharmaceutics The Science of Dosage Form Design Edisi Kedua
Badan Standardisasi Nasional. 2014. Syarat Mutu Kolagen. Jakarta.
Bayhaqi A. 2016. Peranan Minyak Cengkeh (Eugenia aromatica) Pada Transportasi Tertutup Benih Ikan Bandeng (Chanos chanos), http://digilib.umg.ac.id/index.php.
Buckle KE. 2010. Food Science. Jakarta : Penerbit Universitas Indonesia(UI-Press) 18 Tahun 2012 Tentang Pangan 18(9) 1689-1699
Chuaychan S, Benjakul S, Kishimura H. 2015. Characteristics of acid – and pepsin- soluble collagens from scale seabass (Lates calcarifer). LWT – Food Science and Technology . 63(1) : 71-76.
Cui FX, Xue CH, Li ZJ, Zhang YQ, Dong P, Fu XY, Gao X. 2007. Characterization and subunit composition of collagen from the body wall of sea cucumber Stichopus japonicus. The Journal of Food Chemistry 100:1120–1125.
Depkes RI, 1986, Jenis Jenis Ekstraksi, Departemen Kesehatan RI, Jakarta.
Dirjen Perikanan Budidaya KKP RI. 2012. Panduan Penilaian Sertifikasi Cara Budidaya Ikan yang Baik (CBIB). Jakarta
Dokic L, Krstonosic V, Nikolic I. Physicochemical characteristics and stability of oil-in-water emulsions stabilized by OSA starch. Food Hydrocolloids. 2012; 29(1):185–92.
Dybowska B. 2011. Whey protein-stabilized emulsion properties in relation to thermal modification of the continuous phase. Journal of Food Engineering 104(1):81-88
Ennaas N, Hammami R, Gomaa A, Bédard F, Biron É, Subirade M, Fliss I. 2016. Collagencin, an antibacterial peptide from fish collagen: Activity, structure and interaction dynamics with membrane. Biochemical and biophysical research communications, 473(2): 642-647.
Ermawati DE, Martodihardjo S, dan Sulaiman TNS. 2017. Optimasi Komposisi Emulgator Formula Emulsi Air Dalam Minyak Jus Buah Stroberi (Fragaria vesca L.) dengan Metode Simplex Lattice Design. Journal of Pharmaceutical Science and Clinical Research, 02: 78-89.
Fratzl P. 2008, Collagen: Structure and Mechanics, New York: Springer.
Gotanco RGB and Menez MAJ. 2004. Population Genetic Structure of Milkfish, Chanos chanos, Based on PCR-RFLP Analysis of the Mithocondrial Control Region. Marine Biology, 145: 789-801.
Hartati IL, Kurniasari. 2010 Kajian Produksi Kolagen dari Limbah Sisik Ikan Secara Ekstraksi Enzimatis. Momentum Vol. 6, No. 1, April 2010.
Hartayanie L, Adriani M, Lindayani. 2014. Karakteristik Emulsi Santan dan Minyak Kedelai yang ditambah Gum Arab dan Sukrosa Ester. J. Teknol. dan Industri Pangan, 25 (2).
Hoyer B, Bernhardt A, Lode A, Heinemann S, Sewing J, Klinger M, Notbohm H, Gelinsky M. 2014. Jellyfish collagen scaffolds for cartilage tissue engineering. Acta Biomaterialia. 10(2).
Indayanti D. 2014. Uji stabilitas fisik dan komponen kimia pada minyak biji jinten hitam (Nigella sativa L) dalam bentuk emulsi tipe minyak dalam air menggunakan GC-MS. Jakarta : Universitas Islam Negeri Syarif Hidayatullah.
Jafari H, Lista A, Siekapen MM, Ghaari-Bohlouli P, Alimoradi H, Shavandi A. 2020. Review Fish Collagen: Extraction, Characterization, and Applications for Biomaterials Engineering. Polymers, 12: 2230
Jamilah B, Umi Hartina MR, Mat Hashim D, Sazili AQ. 2013. Properties of collagen from barramundi (Lates calcarifer) skin. International Food Reseacrh Journal, 20(2): 835-842.
Kelly S, Duplessis L, Soley J, Noble F, Wells CH, Kelly JP. 2021. Effect Of Preservaties On Corneal Collagen Parameters Measured By Small Angle X-ray Scattering Analysis. 14(17).
Kemenkes RI. 2020. Farmakope Indonesia Edisi VI. Jakarta
Ketnawa S, Martínez-Alvarez O, Benjakul S, Rawdkuen S. 2016. Gelatin hydrolysates from farmed Giant catfish skin using alkaline proteases and its antioxidative function of simulated gastrointestinal digestion. Food chemistry, 192: 34-42.
Kumar KK, Singh S, Chakraborthy S, Das J, Bajaj M, Hemanth V, Nair M, Thota L, Banerjee P. 2019. Recycling fish skin for utilization in food industry as an effective emulsifier and foam stabilizing agent. Turk J Biochem, 44(3): 332–343
Lachman, L., A.H. Lieberman and J.L. Kanig. (2008). Teori dan Praktek Farmasi Industri, Edisi III. UI Press, Jakarta.
León-López A, Vargas-Torres A, Morales-Peñaloza A, Martínez-Juárez VM, Zeugolis DI, dan Aguirre-Álvarez G. 2019. Review Hydrolyzed Collagen—Sources and Applications. Molecules, 24: 4031
Liu H, Li D, Guo S. 2007. Studies on collagen from the skin of channel catfish (Ictalurus punctaus). Food Chemistry. 101.
Mahboob S. 2015. Isolation and characterization of collagen from ?sh waste material-skin, scales and ?ns of Catla catla and Cirrhinus mrigala. J. Food Sci. Technol, 52: 4296–4305
Manikkam V, Mathai ML, Street WA, Donkor ON, Vasiljevic T. 2016. Biofunctional and physicochemical properties of fish scales collagen-derived protein powders. International Food Research Journal, 23(4): 1614-1622.
McClements DJ. Critical review of techniques and methodologies for characterization of emulsion stability. Critical Reviews in Food Sci. and Nutrition. 2007;47(7):611–649.
Mirhosseini H, Tan CP, Hamid NSA, Yusof S, Chern BH. 2009. Characterization of the influence of main emulsion components on the physicochemical properties of orange beverage emulsion using response surface methodology. Food Hydrocolloids, 23(2):271–80.
Mukhriani T. 2014. Ekstraksi, Pemisahan Senyawa, dan Identifikasi Senyawa Aktif. Jurnal Kesehatan, Vol 7, No 2.
Muyonga JH, Cole CGB, Duodu KG. 2004. Extraction and Physico-chemical Characterisation of Nile Perch ( Lates niloticus ) Skin and Bone Gelatin. Journal Food Hydrocolloids. 18: 581-592.
Noomhorm A, Ahmad I, Anal AK. 2014. Functional foods and dietary supplements: Processing effects and health benefits. Wiley & Sons, Ltd. Chichester
Norman W. 1988. Teknologi Pengawetan Pangan. Jakarta : UI Press.
Nurhidayah B, Soekendarsi E, Erviani AE. 2019. Kandungan Kolagen Sisik Ikan Bandeng Chanos-Chanos Dan Sisik Ikan Nila Oreochromis Niloticus. Jurnal Biologi Makassar, 4(1): 39-47.
Pamungkas BF, Supriyadi, Murdiati A, Indrati R. 2018. Ekstraksi dan Karakterisasi Kolagen Larut Asam dan Pepsin dari Sisik Haruan (Channa Striatus) Kering. JPHPI Volume 21 Nomor 3.
Pati F, Adhikari B, Dhara S. 2010. Isolation and characterization of fish scale collagen of higher thermal stability. Bioresource Technology. 101(10).
Paudi R, Sulistijowati R, Mile L. 2020. Rendeman Kolagen Ikan Bandeng (chanos chanos) Segar Hasil Ekstraksi Asam Asetat. Jambura Fish Processing Journal, 2(1)
Pestle W, Colvard DM. 2012. Bone Collagen Preservation In The Tropics A Case Study From AncientPuerto Rico. 39(7).
Ramdhani FG, Ariani A. 2016. Pengambilan Kolagen Pada Sisik Ikan Dari Limbah Pabrik Fillet Ikan Menggunakan Metode Ekstraksi Asam. Institut Teknologi Sepuluh November Surabaya.
Romadhon, Darmanto YS, Kurniasih RA. 2019. Karakteristik Kolagen Dari Tulang, Kulit, Dan Sisik Ikan Nila. JPHPI, 22 (2)
Saanin H. 1984. Taksonomi dan Kunci Identifikasi Ikan, Jilid I-II. Edisi II. Bina Cipta. Bogor.
Saari H, Herafivar K, Rayner M, Wahlgren M, Sjoo M. 2016. Preparation and characterization of starch particles for used in pickering emulsions. Cereal Chemistry. 93(2):116124.
Santana RC, Sato ACK, da Cunha RL. 2012. Emulsions stabilized by heat-treated collagen fibers. Food Hydrocolloids, 26(1): 73-81
Sibilla S, Godfrey M, Brewer S, Budh-Raja A, Genovese L. 2015. An overview of the beneficial effects of hydrolysed collagen as a nutraceutical on skin properties: scientific backround and clinical studies. The Open Nutraceuticals Journals, 8: 29-42
Stansby, ME. 1963. Industrial Fishery Technology. London: Reinhold Publisher Ltd.
Sun C, Gunasekaran S. 2009. Effects of protein concentration and oil-phase volume fraction on the stability and rheology of menhaden oil-in-water emulsions stabilized by whey protein isolate with xanthan gum. Food Hydrocolloids. 23(1):165–174.
Suptijah P, Indriani D, Wardoyo SE. 2018. Isolasi Dan Karakterisasi Kolagen dari Kulit Ikan Patin (Pangasius sp.). Jurnal Sains Natural Vol 8, No. 1.
Suryani, R. Hamsidi & N. Ikawati. 2015. Uji Stabilitas dan Batas Lapisan Emulsi. Prosiding Seminar Nasional Swasembada Pangan. 2:234-241.
Syamsuni, H.A. 2006. Ilmu Resep. Jakarta: Buku Kedokteran EGC
Thakkar, H., Parmar, M., Nangesh, J., & Patel, D. (2011). Formulation And Characterization Of Lipid-Based Drug Delivery System Of Raloxifene-Microemulsion And Self- Microemulsifying Drug Delivery System. Journal of Pharmacy and Bioallied Sciences, 3(3), 442. https://doi.org/10.4103/0975-7406.84463
Toha A, Zulkarnain I, Purnamasari V. 2020. Cream Formulation of Singkong Leaf (Manihot Utilissima) Extract as Antihyperpigmentation with Various Concentration of Emulgators. Jurnal Ilmiah Farmako Bahari, 11(1), Hal 46-56
Veeruraj A, Arumugam M, Balasubramanian T. 2013. Isolation and characterization of thermostable collagen from the marine eel-fish (Evenchelys macrura). Process Biochemistry, 48(10): 1592-1602.
Voigt, R. 1995. Buku Pelajaran Teknologi Farmasi, Edisi V. Yogyakarta: Gadjah Mada Universiti Press
Wahyu IY. 2018. Optimasi Proses Pretreatment Pada Sisik Ikan Bandeng (chanos chanos frskal ) Dengan Response Surface Methodolgy. Prosiding Seminar Nasional Kelautan dan Perikanan IV.
Widria Y, Trilaksani W, Cahyadi ER. 2016. Evaluasi dan Pengembangan Sistem Manajemen Rantai Pasok Bandeng Segar (Chanos chanos) di Kota Bekasi, Jawa Barat. Jurnal MPI, 11(2)
Widya A. wahyu. 2020 . Pengaruh Kecepatan Dan Lama Pengadukan Nanopartikel Buah Parijoto 11 (Medinilla Speciosa) Terhadapan Ukuran Dan Distribusi Partikel, Persen Transmitan Dan Morfologi Partikel
Wulandari. 2015. Efektivitas Pretreatment Alkali dan Hidrolisis Asam Asetat Terhadap Karakteristik Kolagen Dari Kulit Ikan Gabus. 18(3).
Yeon JJ, Karbstein PH, Schaaf DVSU. 2019. Collagen Peptide-Ioaded W1/O Single Emulsions And W1/O/W2 Double Emulsions : Influence Of Collagen Peptide And Salt Concentration, Dispersed Phase Fraction And Type Of Hydrophylic Emulsifier On Droplet Stability And Encapsulation Efficiency.
Zdzieblik D, Oesser S, Gollhofer A, König D. 2017. Improvement of activity-related knee joint discomfort following supplementation of specific collagen peptides. Applied Physiology, Nutrition and Metabolism, 42(6): 588-595.
Zhang J, Duan R, Ye C, Konno K. 2010. Isolation and characterization of collagens from scale of silver carp (Hypophthalmichys molitrix). Journal of Food Biochemistry. 34.
Published
How to Cite
Issue
Section
Copyright (c) 2023 International journal of health & medical sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health & Medical Sciences (IJHMS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHMS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHMS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
