The effectiveness testing of l-carnitine on the quality of spermatozoa and testosterone hormone in white rats (Rattus norvegicus) feeding with high fat


  • Ni Wayan Sukma Antari Institut Teknologi dan Kesehatan Bali
  • Ida Ayu Manik Damayanti Institut Teknologi dan Kesehatan Bali, Indonesia
  • Nadya Treesna Wulansari Institut Teknologi dan Kesehatan Bali, Indonesia


quality of spermatozoa, testosterone L-carnitine hormone, white rats


This study aims to determine the increase in the quality of spermatozoa and testosterone after giving L-carnitine to white rats (Rattus norvegicus). This study was conducted by giving L-carnitine as a treatment for 42 days in white rats with a dose variation of 100 mg/kg bw, 150 mg/kg bw, and 200 mg/kg bw and user controls as a comparison. The variables observed in this study were the quality of spermatozoa, namely: morphology, motility, viability, membrane integrity, and seeing the levels of the hormone testosterone. The research data were processed using a computer statistical program (SPSS 22.0 for Windows) using the One Way Anova test. The results showed that giving high doses of L-carnitine supplements for a long time could cause decreased spermatozoa quality, namely: morphology, motility, viability, membrane integrity and decreased levels of the hormone testosterone.


Download data is not yet available.


Agarwal, A., & Said, T. M. (2004). Carnitines and male infertility. Reproductive biomedicine online, 8(4), 376-384.

Antari, N. W. S., Hayati, A., & Winarni, D. (2016). Provision of Arak Reduces Spermatozoa Quality of White Rats (Rattus norvegicus). Folia Medica Indonesiana, 52(4), 235-240.

Chatzifotis, S., & Takeuchi, T. (1997). Effect of supplemental carnitine on body weight loss, proximate and lipid compositions and carnitine content of red sea bream (Pagrus major) during starvation. Aquaculture, 158(1-2), 129-140.

Chatzifotis, S., Takeuchi, T., & Seikai, T. (1996). The effect of dietary carnitine supplementation on growth of red sea bream (Pagrus major) fingerlings at two levels of dietary lysine. Aquaculture, 147(3-4), 235-248.

Ciereszko, A., Ottobre, J. S., & Glogowski, J. (2000). Effects of season and breed on sperm acrosin activity and semen quality of boars. Animal reproduction science, 64(1-2), 89-96.

Djuwantono, T. (2008). Hanya 7 hari memahami infertilitas. Bandung: PT. Refika Aditama.

Dorado, J., Alcaráz, L., Duarte, N., Portero, J. M., Acha, D., Demyda, S., ... & Hidalgo, M. (2011). Centrifugation on PureSperm® density-gradient improved quality of spermatozoa from frozen-thawed dog semen. Theriogenology, 76(2), 381-385.

Ermayanti, N. G. A. M., Oka, I. G. L., Mahardika, I. G., & Suyadnya, I. P. (2016). Free testosterone level and quality of cauda epididymis sperm of local rabbit that given commercial feed supplemented by cod fish liver oil. International Research Journal of Engineering, IT and Scientific Research, 2(3), 1-8.

Ganong, W.F. 2001. Buku Ajar Fisiologi Kedokteran. Penerbit Buku Kedokteran EGC. Jakarta

Haryati, N. (2003). Pengaruh Suhu dan Lama Penyimpanan Sosis Daging Sapi Terhadap Total Bakteri dan Penilaian Organoleptik (Doctoral dissertation, Diponegoro University).

Hayati, A. (2011). Spermatologi. Surabaya: Pusat Penerbitan dan Percetakan Unair.

Hoek, E., Carranza-Torres, C., & Corkum, B. (2002). Hoek-Brown failure criterion-2002 edition. Proceedings of NARMS-Tac, 1(1), 267-273.

Jacyno, E., Ko?odziej, A., Kamyczek, M., Kaw?cka, M., Dziadek, K., & Pietruszka, A. (2007). Effect of L-carnitine supplementation on boar semen quality. Acta Veterinaria Brno, 76(4), 595-600.

Karadeniz, A., Simsek, N., & Cakir, S. (2008). Haematological effects of dietary L-carnitine supplementation in broiler chickens. Revue Méd. Vét, 159(8-9), 437-444.

Matalliotakis, I. & Koumantakis, E. (2000). L-carnitine levels in the seminal plasma of fertile and infertile men: correlation with sperm quality. Int. J. Fert. Womens Med. 45, 236-240.

Mohammadi, V., Sharifi, S. D., Sharafi, M., Mohammadi-Sangcheshmeh, A., Shahverdi, A., & Alizadeh, A. (2021). Manipulation of fatty acid profiles in roosters’ testes, alteration in sexual hormones, improvements in testicular histology characteristics and elevation sperm quality factor by L-carnitine. Theriogenology, 161, 8-15.

Murray, G. M. (2007). Acrosome size and kinematics of human spermatozoa (Doctoral dissertation, Stellenbosch: University of Stellenbosch).

Neuman, S. L., Lin, T. L., & Heste, P. Y. (2002). The effect of dietary carnitine on semen traits of White Leghorn roosters. Poultry Science, 81(4), 495-503.

NG, C. M., Blackman, M. R., Wang, C., & Swerdloff, R. S. (2004). The role of carnitine in the male reproductive system. Annals of the New York Academy of Sciences, 1033(1), 177-188.

O'shaughnessy, P. J., Monteiro, A., Verhoeven, G., De Gendt, K., & Abel, M. H. (2010). Effect of FSH on testicular morphology and spermatogenesis in gonadotrophin-deficient hypogonadal mice lacking androgen receptors. Reproduction (Cambridge, England), 139(1), 177.

Owen, K. Q., Jit, H., Maxwell, C. V., Nelssen, J. L., Goodband, R. D., Tokach, M. D., ... & Koo, S. I. (2001). Dietary L-carnitine suppresses mitochondrial branched-chain keto acid dehydrogenase activity and enhances protein accretion and carcass characteristics of swine. Journal of Animal Science, 79(12), 3104-3112.

Ri, K. (2013). Riset kesehatan dasar. Jakarta: Badan Penelitian dan pengembangan Kesehatan Kementrian Kesehatan RI.

Sarica, M., Ocak, N., Karacay, N., Yamak, U., Kop, C. A. N. A. N., & Altop, A. (2009). Growth, slaughter and gastrointestinal tract traits of three turkey genotypes under barn and free-range housing systems. British Poultry Science, 50(4), 487-494.

Sherwood, L. (2011). Fisiologi manusia dari sel ke sistem edisi 6. Alih bahasa: brahm U. Editor Nella Yesdelita. Jakarta: EGC.

Suharyati, S., & Hartono, M. (2013). Peningkatan kualitas semen kambing Boer dengan pemberian Vitamin E dan Mineral Zn. Jurnal Kedokteran Hewan-Indonesian Journal of Veterinary Sciences, 7(2).

Sundseth, S. S., & Waxman, D. J. (1992). Sex-dependent expression and clofibrate inducibility of cytochrome P450 4A fatty acid omega-hydroxylases. Male specificity of liver and kidney CYP4A2 mRNA and tissue-specific regulation by growth hormone and testosterone. Journal of Biological Chemistry, 267(6), 3915-3921.

Vaz, G., Roussouly, P., Berthonnaud, E., & Dimnet, J. (2002). Sagittal morphology and equilibrium of pelvis and spine. European spine journal, 11(1), 80-87.

Vicari, E., & Calogero, A. E. (2001). Effects of treatment with carnitines in infertile patients with prostato-vesiculo-epididymitis. Human Reproduction, 16(11), 2338-2342.



How to Cite

Antari, N. W. S. ., Damayanti, I. A. M. ., & Wulansari, N. T. . (2021). The effectiveness testing of l-carnitine on the quality of spermatozoa and testosterone hormone in white rats (Rattus norvegicus) feeding with high fat. International Journal of Health & Medical Sciences, 4(1), 102-109.



Peer Review Articles