Resistance test of several varieties and critical phase for cucumis sativus towards cucumber mosaic virus infectio

Authors

  • Ni Putu Pandawani Mahasaraswati University, Denpasar, Bali-Indonesia
  • Farida Hanum Mahasaraswati University, Denpasar, Bali-Indonesia
  • Ni Nyoman Suryani Mahasaraswati University, Denpasar, Bali-Indonesia

Keywords:

Cucumber, Critical Phase, Resistant Varieties, Cucumber Mosaic Virus (CMV)

Abstract

The present study was intended to obtain resistant cucumber varieties and to know the critical phase of cucumber plant towards Cucumber Mosaic Virus infection (CMV) therefore, it could be used as a basis for controlling the mosaic disease spread of CMV-induced at cucumber plants. The research was conducted in Baturiti Village Tabanan Bali within an insect-proof greenhouse. Elisa test was performed using CMV, WMV and PRsV antiserum. The resistance test of several cucumber varieties included Roberto, Harmony, Citra baby and Manggala was conducted by mechanical transmission from CMV inoculum source sap. Testing of cucumber plant critical phase of CMV infection was done by mechanically inoculating sap from CMV infected plant, for healthy cucumber plant as testing plant i.e. inoculation at 2, 3, 4, 5 and 6 weeks after planting and control (plant without CMV inoculation). An observation was done for incubation period variables, disease incidence, disease severity and crop yield. The results and conclusions of the four cucumber varieties tested were not found to be CMV resistant varieties, however, the moderately sensitive Harmony varieties deserve attention for choosing in the cultivation. The cucumber plant that was CMV infected from the age at planting until 4 weeks after planting shows 100% disease incidence and plants could not produce fruits. The critical phase of cucumber plant towards CMV infection occurred from the time at planting until 4 weeks after planting.

Downloads

Download data is not yet available.

References

Agrios, G. N. (2005). Plant Pathology. 5th eds. Department of Plant Pathology. University of Florida. United States of America.

Akhtar, K. P., Hussain, M., Khan, A. I., Haq, M. A., & Iqbal, M. M. (2004). Influence of plant age, whitefly population and cultivar resistance on infection of cotton plants by cotton leaf curl virus (CLCuV) in Pakistan. Field crops research, 86(1), 15-21. https://doi.org/10.1016/S0378-4290(03)00166-7

Babadoost, M. (2000). Outbreak of Phytophthora foliar blight and fruit rot in processing pumpkin fields in Illinois. Plant disease, 84(12), 1345-1345. https://doi.org/10.1094/PDIS.2000.84.12.1345A

Balitsa. (2006). Guidelines for the Introduction and Control of Viral Diseases in Chili. Indonesian Vegetable Research Institute, Center for Horticulture research and development. Agency for Agricultural Research and Development. Lembang-Bandung.

Bananej, K., & Vahdat, A. (2008). Identification, distribution and incidence of viruses in field-grown cucurbit crops of Iran. Phytopathologia Mediterranea, 47(3), 247-257.

Djikstra, J., & Jagger, D. (1998). Practical Plant Virology: Protocol and Exercise.

Dolores, L. M., & Valdez, R. B. (1988). Identification of squash viruses and screening for resistance. Philippine Phytopathology (Philippines).

Hadiastono, T. (1998). Plant Virology. Identification and Diagnosis of Plant Virology. Faculty of Agriculture Universitas Brawijaya, Malang.

Hemida, S. K. (2005). Effect of bean yellow mosaic virus on physiological parameters ofVicia faba and Phaseolus vulgaris. International Journal of Agriculture and Biology (Pakistan).

Hull, R. (2013). Plant virology. Academic press.

Ko, S. J., Lee, Y. H., Cha, K. H., Lee, S. H., Choi, H. S., Choi, Y. S., ... & Kim, K. H. (2006). Incidence and distribution of virus diseases on cucumber in Jeonnam Province during 1999-2002. The Plant Pathology Journal, 22(2), 147-151.

Mandal, B., Majumder, B., Bandyopadhyay, P. K., Hazra, G. C., Gangopadhyay, A., Samantaray, R. N., ... & Kundu, S. (2007). The potential of cropping systems and soil amendments for carbon sequestration in soils under long‐term experiments in subtropical India. Global change biology, 13(2), 357-369. https://doi.org/10.1111/j.1365-2486.2006.01309.x

Matthews, R. E. F. (2002). Hull R. Matthews' plant virology.

Melton III, L. J., Thamer, M., Ray, N. F., Chan, J. K., Chesnut III, C. H., Einhorn, T. A., ... & Siris, E. S. (1997). Fractures attributable to osteoporosis: report from the National Osteoporosis Foundation. Journal of Bone and Mineral Research, 12(1), 16-23.

Müller, C., Bröther, H., Von Bargen, S., & Büttner, C. (2006). Zucchini yellow mosaic virus—incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany. Journal of Plant Diseases and Protection, 113(6), 252-258. https://doi.org/10.1007/BF03356190

Provvidenti, R. (1986). Viral diseases of cucurbits and sources of resistance. Plant virus diseases of horticultural crops in the tropics and subtropics.

Sumpena, U. (2012). Respon beberapa kultivar mentimun terhadap zymv (zucchini yellow mosaic virus). Mediagro, 8(2).

Published

2017-11-07

How to Cite

Pandawani, N. P., Hanum, F., & Suryani, N. N. (2017). Resistance test of several varieties and critical phase for cucumis sativus towards cucumber mosaic virus infectio. International Research Journal of Engineering, IT & Scientific Research, 3(6), 68–76. Retrieved from https://sloap.org/journals/index.php/irjeis/article/view/11

Issue

Section

Research Articles