The number of leydig cells, sertoli cells, and spermatogonia are lower towards a little rats that their parent given genistein during periconception period
Keywords:
Genistein, Leydig cell, Periconception, Sertoli cell, SpermatogoniaAbstract
The testis was composed by the cells of Leydig, Sertoli, and Spermatogenic. The cells formation itself was able to be disrupted by exposure to an endocrine disrupting chemical (EDC) since the prenatal period. The research was intended to prove that Genistein could obstruct the formation of Leydig cells, Sertoli cells, Spermatogenic of rats. The randomized post-test only control group design method was conducted towards white female Wistar rats, 12-13 weeks old, has been had one child, could normally eat and drink. The analysis unit was the child of mother rat treatment group that given Genistein of 10 mg/kg/day and the control group that received distilled water, each of them were 15. The research was done in the Laboratory of Faculty of Veterinary Medical, Udayana University, from January to July 2016. The computer was used for analyzing the data using, with ? of 0.05. The result was the Genistein child had an average of 5.464 Sertoli cell, 11.120 Leydig cell, and 48.427 spermatogonia, whereas, the control group had an average of 8.173 Leydig cells, 12.987 Sertoli cells, and 69.547 spermatogonia. There were significant differences between the two groups (p 0.000). The conclusion was that an average of Leydig cells, Sertoli cells, and Spermatogonia lower in children whose their parent rats given Genistein during periconception period.
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References
Barbieri, R. L. (2014). The endocrinology of the menstrual cycle. In Human Fertility (pp. 145-169). Humana Press, New York, NY.
Bucar, F. (2012). Phytoestrogens in plants: with special reference to isoflavones. Isoflavones, Chemistry, Analysis, Function and Effects, 14-27.
Casanova, M., You, L., Gaido, K. W., Archibeque-Engle, S., Janszen, D. B., & Heck, H. D. A. (1999). Developmental effects of dietary phytoestrogens in Sprague-Dawley rats and interactions of genistein and daidzein with rat estrogen receptors alpha and beta in vitro. Toxicological sciences: an official journal of the Society of Toxicology, 51(2), 236-244.
Craig, Z. R., Wang, W., & Flaws, J. A. (2011). Endocrine disrupting chemicals in ovarian function: effects on steroidogenesis, metabolism and nuclear receptor signaling. Reproduction, REP-11.
Delbès, G., Levacher, C., & Habert, R. (2006). Estrogen effects on fetal and neonatal testicular development. Reproduction, 132(4), 527-538.
Erb, C. (2006). Embryology and teratology. In The Laboratory Rat (Second Edition) (pp. 817-846).
Ernst, L. M., Ruchelli, E. D., & Huff, D. S. (Eds.). (2011). Color atlas of fetal and neonatal histology. Springer Science & Business Media.
Franke, A. A., Halm, B. M., Custer, L. J., Tatsumura, Y., & Hebshi, S. (2006). Isoflavones in breastfed infants after mothers consume soy–. The American journal of clinical nutrition, 84(2), 406-413.
Kim, S. H., & Park, M. J. (2012). Effects of phytoestrogen on sexual development. Korean journal of pediatrics, 55(8), 265-271.
Larkin, T., Price, W. E., & Astheimer, L. (2008). The key importance of soy isoflavone bioavailability to understanding health benefits. Critical reviews in food science and nutrition, 48(6), 538-552.
Lofamia, E. A. A., Ramos, G. B., Mamon, M. A. C., Salido, F. M., Su, G. S., & de Vera, M. P. (2014). Isoflavone maternal-supplementation during periconception period: Influence on the reproductive organs of the first generation (f1) murine weanling-stage offspring. Asian Pacific Journal of Reproduction, 3(4), 268-274.
Patisaul, H. B., Blum, A., Luskin, J. R., & Wilson, M. E. (2005). Dietary soy supplements produce opposite effects on anxiety in intact male and female rats in the elevated plus-maze. Behavioral neuroscience, 119(2), 587.
Retana-MÃ, S., Muñoz-GutiÃ, M., Duarte, G., Vielma, J., Fitz-RodrÃguez, G., & Keller, M. (2011). Effects of phytoestrogens on mammalian reproductive physiology. Tropical and Subtropical Agroecosystems, 15(S1).
Sadler, T. W. (2005, May). Embryology of neural tube development. In American Journal of Medical Genetics Part C: Seminars in Medical Genetics (Vol. 135, No. 1, pp. 2-8). Hoboken: Wiley Subscription Services, Inc., A Wiley Company.
Speroff, L., & Fritz, M. A. (Eds.). (2005). Clinical gynecologic endocrinology and infertility. lippincott Williams & wilkins.
Todaka, E., Sakurai, K., Fukata, H., Miyagawa, H., Uzuki, M., Omori, M., ... & Mori, C. (2005). Fetal exposure to phytoestrogens—the difference in phytoestrogen status between mother and fetus. Environmental research, 99(2), 195-203.
Uzumcu, M., Zama, A. M., & Oruc, E. (2012). Epigenetic mechanisms in the actions of endocrine‐disrupting chemicals: gonadal effects and role in female reproduction. Reproduction in domestic animals, 47, 338-347.
Weinbauer, G. F., Luetjens, C. M., Simoni, M., & Nieschlag, E. (2010). Physiology of testicular function. In Andrology (pp. 11-59). Springer, Berlin, Heidelberg.
World Health Organization. (2013). Oral health surveys: basic methods. World Health Organization.
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